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Contributor submission ApyS #918

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mite-bot Sep 19, 2026
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mite-bot Sep 19, 2026
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55 changes: 55 additions & 0 deletions mite_data/data/d47cb808-b45f-11f1-b4b1-cf84655b59e7.json
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{
"accession": "MITE9999999",
"status": "pending",
"comment": "ApyS catalyzes methylation of the \u03b2-carbon of the aminopyruvic acid to complete the C-terminal \u03b2-methylated aminopyruvate group during aminopyruvatide biosynthesis.",
"changelog": [
{
"version": "1",
"date": "2026-09-19",
"contributors": [
"0009-0008-8086-5325"
],
"reviewers": [
"BBBBBBBBBBBBBBBBBBBBBBBB"
],
"comment": "New entry."
}
],
"enzyme": {
"name": "ApyS",
"description": "Methyltransferase",
"references": [
"doi:10.1021/acscentsci.4c00088"
],
"databaseIds": {
"uniprot": "UPI0000667567",
"genpept": "ABC34725.1"
}
},
"reactions": [
{
"tailoring": [
"Methylation"
],
"description": "ApyS catalyzes methylation of the \u03b2-carbon of the aminopyruvic acid generated by ApyH/ApyI to form a C-terminal 3-amino-2-oxobutanoic acid",
"reactionSMARTS": 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"reactions": [
{
"substrate": "CCC(C)C(NC(=O)C(C)NC(=O)C(CCCCN)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C(C)NC(=O)C(CC(=O)O)NC(=O)C(NC(=O)C1CCCN1C(=O)C(CCSC)NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCSC)NC(=O)C(CCCCN)NC(=O)C1CCCN1C(=O)C(CC(C)C)NC(=O)C(CCCCN)NC(=O)CNC(=O)C(CCC(=O)O)NC(=O)C(NC(=O)C(CO)NC(=O)C(NC(=O)C(CO)NC(=O)C(NC(=O)CNC(=O)C(CCCCN)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C1CCCN1C(=O)C(CCCCN)NC(=O)C(NC(=O)C(C)NC(=O)C(N)CCSC)C(C)O)C(C)O)C(C)C)C(C)C)C(C)CC)C(C)O)C(C)C)C(C)CC)C(=O)NC(CCC(N)=O)C(=O)NC(CCCCN)C(=O)NC(CS)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(N)=O)C(=O)NCC(=O)NC(CCCCN)C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(CCSC)C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC(=O)O)C(=O)NC(CC(C)C)C(=O)NC(C)C(=O)NCC(=O)NCC(=O)NC(CCCNC(=N)N)C(=O)NC(CCSC)C(=O)NCC(=O)NC(CCC(=O)O)C(=O)NCC(=O)NC1Cc2ccc(O)c(c2)-c2cc(ccc2O)C(C)C(C(=O)NCC(=O)C(=O)O)NC(=O)C(CC(C)C)NC1=O)C(C)C)C(C)O)C(C)CC)C(C)O",
"products": [
"CCC(C)C(NC(=O)C(C)NC(=O)C(CCCCN)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C(C)NC(=O)C(CC(=O)O)NC(=O)C(NC(=O)C1CCCN1C(=O)C(CCSC)NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCSC)NC(=O)C(CCCCN)NC(=O)C1CCCN1C(=O)C(CC(C)C)NC(=O)C(CCCCN)NC(=O)CNC(=O)C(CCC(=O)O)NC(=O)C(NC(=O)C(CO)NC(=O)C(NC(=O)C(CO)NC(=O)C(NC(=O)CNC(=O)C(CCCCN)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C1CCCN1C(=O)C(CCCCN)NC(=O)C(NC(=O)C(C)NC(=O)C(N)CCSC)C(C)O)C(C)O)C(C)C)C(C)C)C(C)CC)C(C)O)C(C)C)C(C)CC)C(=O)NC(CCC(N)=O)C(=O)NC(CCCCN)C(=O)NC(CS)C(=O)NC(CC(C)C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(N)=O)C(=O)NCC(=O)NC(CCCCN)C(=O)NC(CC(C)C)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(CCSC)C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC(=O)O)C(=O)NC(CC(C)C)C(=O)NC(C)C(=O)NCC(=O)NCC(=O)NC(CCCNC(=N)N)C(=O)NC(CCSC)C(=O)NCC(=O)NC(CCC(=O)O)C(=O)NCC(=O)NC1Cc2ccc(O)c(c2)-c2cc(ccc2O)C(C)C(C(=O)NC(C)C(=O)C(=O)O)NC(=O)C(CC(C)C)NC1=O)C(C)C)C(C)O)C(C)CC)C(C)O"
],
"isIntermediate": false
}
],
"evidence": {
"evidenceCode": [
"Heterologous expression"
],
"references": [
"doi:10.1021/acscentsci.4c00088"
]
}
}
]
}
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