Contributor submission eryF

mite_data/data/MITE0000502.json

@@ -0,0 +1,68 @@
+{
+    "accession": "MITE0000502",
+    "status": "active",
+    "comment": "Catalyzes the conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB.",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2026-07-14",
+            "contributors": [
+                "0000-0003-3002-6458"
+            ],
+            "reviewers": [
+                "0000-0002-4540-6501"
+            ],
+            "comment": "New entry. Reviewer 1 changed Description"
+        }
+    ],
+    "enzyme": {
+        "name": "EryF",
+        "description": "Cytochrome P450",
+        "references": [
+            "doi:10.1021/bi00059a004"
+        ],
+        "databaseIds": {
+            "uniprot": "Q5Y9H2",
+            "genpept": "AAU93799.1",
+            "mibig": "BGC0000054"
+        },
+        "cofactors": {
+            "organic": [
+                "Heme",
+                "NADP"
+            ],
+            "inorganic": [
+                "Fe"
+            ]
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Hydroxylation",
+                "Monooxygenation"
+            ],
+            "description": "Catalyzes the conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the P450 monooxygenase",
+            "reactionSMARTS": "[#6@:1]1(/[#8:23])-[#6@@:14](\\[#6:17])-[#6@@:13](\\[#6:18]-[#6:26])-[#8:12]-[#6:11](=[#8:25])-[#6@:10](/[#6:21])-[#6@@:9](\\[#8:20])-[#6:8]-[#6@@:7](\\[#8:19])-[#6@@:6](\\[#6:16])-[#6:5]-[#6@@:4](\\[#6:15])-[#6:3](=[#8:24])-[#6@@:2]-1/[#6:22]>>[#6@:1]1(/[#8:23])-[#6@@:14](\\[#6:17])-[#6@@:13](\\[#6:18]-[#6:26])-[#8:12]-[#6:11](=[#8:25])-[#6@:10](/[#6:21])-[#6@@:9](\\[#8:20])-[#6:8]-[#6@@:7](\\[#8:19])-[#6@@:6](/[#8])(\\[#6:16])-[#6:5]-[#6@@:4](\\[#6:15])-[#6:3](=[#8:24])-[#6@@:2]-1/[#6:22]",
+            "reactions": [
+                {
+                    "substrate": "CC[C@H]1OC(=O)[C@H](C)[C@@H](O)C[C@@H](O)[C@@H](C)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@H]1C",
+                    "products": [
+                        "CC[C@H]1OC(=O)[C@H](C)[C@@H](O)C[C@@H](O)[C@](C)(O)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@H]1C"
+                    ],
+                    "isIntermediate": false
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "Knock-out studies"
+                ],
+                "references": [
+                    "doi:10.1021/bi00059a004",
+                    "doi:10.1126/science.2011746"
+                ]
+            }
+        }
+    ]
+}