Contributor submission SbzM

mite_data/data/MITE0000481.json

@@ -0,0 +1,77 @@
+{
+    "accession": "MITE0000481",
+    "status": "retired",
+    "retirementReasons": [
+        "Not a tailoring enzyme."
+    ],
+    "comment": "Catalyzes the two-step oxidation and decarboxylation of L-cysteine",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2026-04-13",
+            "contributors": [
+                "0000-0003-0455-2227"
+            ],
+            "reviewers": [
+                "0000-0001-6534-6609",
+                "0000-0003-4819-2251"
+            ],
+            "comment": "New entry. Reviewer 1 retired entry."
+        }
+    ],
+    "enzyme": {
+        "name": "SbzM",
+        "description": "Cupin dioxygenase",
+        "references": [
+            "doi:10.1038/s41929-026-01493-z",
+            "doi:10.1038/s41467-018-08093-x"
+        ],
+        "databaseIds": {
+            "uniprot": "P0DY49",
+            "genpept": "BBG06562.1",
+            "mibig": "BGC0001925"
+        },
+        "cofactors": {
+            "inorganic": [
+                "Ni",
+                "Zn"
+            ]
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Decarboxylation",
+                "Oxidation"
+            ],
+            "description": "Two step oxidation and decarboxylation of L-cysteine with an amide transfer to the thiol group.",
+            "reactionSMARTS": "[#7:2]-[#6:1](/[#6:6]-[#16:7])-[#6:3](=[#8:4])-[#8:5].[#8:8]=[#8:9].[#8:10]=[#8:11].[#8:12]>>[#8:12]=[#6:1]-[#6:6]-[#16:7](=[#8:8])(-[#7:2])=[#8:10].[#8:4]=[#6:3]=[#8:5].[#8:9].[#8:11]",
+            "reactions": [
+                {
+                    "substrate": "N[C@@H](CS)C(=O)O.O.O=O.O=O",
+                    "products": [
+                        "NS(=O)(=O)CC=O",
+                        "O",
+                        "O",
+                        "O=C=O"
+                    ],
+                    "isIntermediate": true
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "In vitro assay",
+                    "Site-directed mutagenesis"
+                ],
+                "references": [
+                    "doi:10.1038/s41929-026-01493-z"
+                ]
+            },
+            "databaseIds": {
+                "rhea": "81871",
+                "ec": "1.13.11.95"
+            }
+        }
+    ]
+}