Add workflow for segmentation and counting of cell nuclei in fluorescence microscopy images#345
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mvdbeek merged 6 commits intogalaxyproject:mainfrom Feb 29, 2024
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Looks great, thanks so much @kostrykin! Yes, planemo lint isn't as smart as the best practice panel, I've got some work in galaxyproject/planemo#1417 to improve that.
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As part of the Imaging Hackathon in Freiburg.
Add workflow for segmentation and counting of cell nuclei in fluorescence microscopy images.
This workflow performs segmentation and counting of cell nuclei using fluorescence microscopy images. The segmentation step is performed using Otsu thresholding (Otsu, 1979).
Created by following the tutorial: https://github.com/galaxyproject/iwc/blob/main/workflows/README.md#adding-workflows
Strangely,
planemo workflow_lintreports a couple of issues:…but this workflow step is the input of the workflow, so it cannot be connected to anything.
There also are a few more warnings, which I suspect to be consequential errors, since the Best Practices check in galaxy reports that everything is fine with the workflow:

Let me know if anything needs to be done about those warnings!