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Original file line number Diff line number Diff line change
@@ -1,5 +1,16 @@
# Changelog

## [0.1.2] - 2026-04-20

### Automatic update
- `toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/1.1.0+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/1.3.2+galaxy0`
- `toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff_build_gb/5.2+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff_build_gb/5.4+galaxy0`
- `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.22+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.22+galaxy2`
- `toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.8` was updated to `toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.9`
- `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.33+galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.33+galaxy3`
- `toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff/5.2+galaxy1` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff/5.4+galaxy0`
- `toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/4.3+t.galaxy0` was updated to `toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/5.4.0c+galaxy0`

## [0.1.1] 2026-03-12

- Rename workflow file to remove '+' character from filename.
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Original file line number Diff line number Diff line change
Expand Up @@ -50,10 +50,10 @@
{
"child_steps": [
4,
8,
7,
10,
6,
8,
9
],
"color": "blue",
Expand Down Expand Up @@ -86,7 +86,6 @@
],
"format-version": "0.1",
"license": "MIT",
"release": "0.1.1",
"name": "Paired end variant and ploidy-aware genotype calling",
"readme": "This workflow performs end-to-end germline variant and genotype calling on paired-end Illumina sequencing data from organisms of any given ploidy. Starting from raw FASTQ reads, the workflow handles quality control, read mapping, duplicate removal, variant and genotype calling with FreeBayes, and functional annotation of the resulting variants.",
"report": {
Expand Down Expand Up @@ -203,7 +202,7 @@
},
"4": {
"annotation": "Processing of FASTQ files",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/1.1.0+galaxy0",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/1.3.2+galaxy0",
"errors": null,
"id": 4,
"input_connections": {
Expand Down Expand Up @@ -245,16 +244,16 @@
"output_name": "report_json"
}
},
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/1.1.0+galaxy0",
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/fastp/fastp/1.3.2+galaxy0",
"tool_shed_repository": {
"changeset_revision": "ff33957bf802",
"changeset_revision": "241decaaff3e",
"name": "fastp",
"owner": "iuc",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"duplicated_reads\": {\"handling_options\": {\"eval_dups\": \"--dont_eval_duplication\", \"__current_case__\": 0}}, \"filter_options\": {\"quality_filtering_options\": {\"disable_quality_filtering\": false, \"qualified_quality_phred\": null, \"unqualified_percent_limit\": null, \"n_base_limit\": null}, \"length_filtering_options\": {\"disable_length_filtering\": false, \"length_required\": null, \"length_limit\": null}, \"low_complexity_filter\": {\"enable_low_complexity_filter\": false, \"complexity_threshold\": null}}, \"output_options\": {\"report_html\": true, \"report_json\": true}, \"overrepresented_sequence_analysis\": {\"overrepresentation_analysis\": false, \"overrepresentation_sampling\": null}, \"read_mod_options\": {\"polyg_tail_trimming\": {\"trimming_select\": \"\", \"__current_case__\": 1, \"poly_g_min_len\": null}, \"polyx_tail_trimming\": {\"polyx_trimming_select\": \"\", \"__current_case__\": 1}, \"umi_processing\": {\"umi\": false, \"umi_loc\": null, \"umi_len\": null, \"umi_prefix\": null}, \"cutting_by_quality_options\": {\"cut_front_select\": {\"cut_front\": \"\", \"__current_case__\": 1}, \"cut_tail_select\": {\"cut_tail\": \"\", \"__current_case__\": 1}, \"cut_right_select\": {\"cut_right\": \"\", \"__current_case__\": 1}}, \"base_correction_options\": {\"correction\": false}}, \"single_paired\": {\"single_paired_selector\": \"paired_collection\", \"__current_case__\": 1, \"paired_input\": {\"__class__\": \"ConnectedValue\"}, \"merge_reads\": {\"merge\": \"\", \"__current_case__\": 1}, \"adapter_trimming_options\": {\"disable_adapter_trimming\": false, \"adapter_sequence1\": null, \"adapter_sequence2\": null, \"detect_adapter_for_pe\": false}, \"global_trimming_options\": {\"trim_front1\": null, \"trim_tail1\": null, \"trim_front2\": null, \"trim_tail2\": null}}, \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "1.1.0+galaxy0",
"tool_version": "1.3.2+galaxy0",
"type": "tool",
"uuid": "e1359474-a019-4a41-963b-ff2660bd36cc",
"when": null,
Expand All @@ -268,7 +267,7 @@
},
"5": {
"annotation": "Build SnpEff database from the given reference fasta file",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff_build_gb/5.2+galaxy1",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff_build_gb/5.4+galaxy0",
"errors": null,
"id": 5,
"input_connections": {
Expand Down Expand Up @@ -300,16 +299,16 @@
"top": 828.7082651499238
},
"post_job_actions": {},
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff_build_gb/5.2+galaxy1",
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff_build_gb/5.4+galaxy0",
"tool_shed_repository": {
"changeset_revision": "3aae4f16ac9e",
"changeset_revision": "908f2c5fedec",
"name": "snpeff",
"owner": "iuc",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"codon_table\": \"Standard\", \"genome_version\": \"snpeff_db\", \"input_type\": {\"input_type_selector\": \"gtf\", \"__current_case__\": 2, \"input\": {\"__class__\": \"ConnectedValue\"}, \"reference_source\": {\"reference_source_selector\": \"history\", \"__current_case__\": 1, \"input_fasta\": {\"__class__\": \"ConnectedValue\"}}}, \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "5.2+galaxy1",
"tool_version": "5.4+galaxy0",
"type": "tool",
"uuid": "b717154e-57de-4980-ba7a-0fdd88765ff9",
"when": null,
Expand Down Expand Up @@ -370,7 +369,7 @@
},
"7": {
"annotation": "Retains alignments if read is paired and mapped in proper orientation ",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.22+galaxy1",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.22+galaxy2",
"errors": null,
"id": 7,
"input_connections": {
Expand Down Expand Up @@ -401,24 +400,24 @@
"output_name": "outputsam"
}
},
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.22+galaxy1",
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/samtools_view/samtools_view/1.22+galaxy2",
"tool_shed_repository": {
"changeset_revision": "5ec526530725",
"changeset_revision": "5cf9fcc93508",
"name": "samtools_view",
"owner": "iuc",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"addref_cond\": {\"addref_select\": \"no\", \"__current_case__\": 0}, \"input\": {\"__class__\": \"ConnectedValue\"}, \"mode\": {\"outtype\": \"selected_reads\", \"__current_case__\": 1, \"filter_config\": {\"cond_region\": {\"select_region\": \"no\", \"__current_case__\": 0}, \"cond_rg\": {\"select_rg\": \"no\", \"__current_case__\": 0}, \"cond_expr\": {\"select_expr\": \"no\", \"__current_case__\": 0}, \"quality\": null, \"library\": null, \"cigarcons\": null, \"inclusive_filter\": [\"1\", \"2\"], \"exclusive_filter\": null, \"exclusive_filter_all\": null, \"tag\": null, \"qname_file\": {\"__class__\": \"RuntimeValue\"}}, \"subsample_config\": {\"subsampling_mode\": {\"select_subsample\": \"fraction\", \"__current_case__\": 0, \"factor\": \"1.0\", \"seed\": null}}, \"output_options\": {\"reads_report_type\": \"retained\", \"__current_case__\": 0, \"complementary_output\": false, \"adv_output\": {\"readtags\": [], \"collapsecigar\": false}, \"output_format\": {\"oformat\": \"bam\", \"__current_case__\": 2}}}, \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "1.22+galaxy1",
"tool_version": "1.22+galaxy2",
"type": "tool",
"uuid": "49a309e1-22a6-455a-9fb3-77aa3f5decf4",
"when": null,
"workflow_outputs": []
},
"8": {
"annotation": "Generates statistics of BAM file",
"content_id": "toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.8",
"content_id": "toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.9",
"errors": null,
"id": 8,
"input_connections": {
Expand All @@ -441,16 +440,16 @@
"top": 133.89804204944727
},
"post_job_actions": {},
"tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.8",
"tool_id": "toolshed.g2.bx.psu.edu/repos/devteam/samtools_stats/samtools_stats/2.0.9",
"tool_shed_repository": {
"changeset_revision": "57934e90e27c",
"changeset_revision": "8fca6e1eac98",
"name": "samtools_stats",
"owner": "devteam",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"addref_cond\": {\"addref_select\": \"no\", \"__current_case__\": 0}, \"cond_region\": {\"select_region\": \"no\", \"__current_case__\": 0}, \"cov_threshold\": null, \"coverage_cond\": {\"coverage_select\": \"no\", \"__current_case__\": 0}, \"filter_by_flags\": {\"filter_flags\": \"nofilter\", \"__current_case__\": 1}, \"gc_depth\": null, \"input\": {\"__class__\": \"ConnectedValue\"}, \"insert_size\": null, \"most_inserts\": null, \"read_group\": null, \"read_length\": null, \"remove_dups\": false, \"remove_overlaps\": false, \"sparse\": false, \"split_output_cond\": {\"split_output_selector\": \"no\", \"__current_case__\": 0}, \"trim_quality\": null, \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "2.0.8",
"tool_version": "2.0.9",
"type": "tool",
"uuid": "a0216ddc-23e6-40e3-9088-f3934da46aa1",
"when": null,
Expand Down Expand Up @@ -502,7 +501,7 @@
},
"10": {
"annotation": "Aggregation of results from mapping, and other preprocessing steps",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.33+galaxy0",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.33+galaxy3",
"errors": null,
"id": 10,
"input_connections": {
Expand Down Expand Up @@ -555,16 +554,16 @@
"output_name": "html_report"
}
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"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.33+galaxy0",
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.33+galaxy3",
"tool_shed_repository": {
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"changeset_revision": "862943c10425",
"name": "multiqc",
"owner": "iuc",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"comment\": \"\", \"export\": false, \"flat\": false, \"image_content_input\": {\"__class__\": \"RuntimeValue\"}, \"png_plots\": false, \"results\": [{\"__index__\": 0, \"software_cond\": {\"software\": \"fastp\", \"__current_case__\": 7, \"input\": {\"__class__\": \"ConnectedValue\"}}}, {\"__index__\": 1, \"software_cond\": {\"software\": \"samtools\", \"__current_case__\": 24, \"output\": [{\"__index__\": 0, \"type\": {\"type\": \"stats\", \"__current_case__\": 0, \"input\": {\"__class__\": \"ConnectedValue\"}}}]}}, {\"__index__\": 2, \"software_cond\": {\"software\": \"picard\", \"__current_case__\": 17, \"output\": [{\"__index__\": 0, \"type\": \"markdups\", \"input\": {\"__class__\": \"ConnectedValue\"}}]}}], \"title\": \"\", \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_state": "{\"comment\": \"\", \"export\": false, \"flat\": false, \"image_content_input\": {\"__class__\": \"RuntimeValue\"}, \"png_plots\": false, \"results\": [{\"__index__\": 0, \"software_cond\": {\"software\": \"fastp\", \"__current_case__\": 8, \"input\": {\"__class__\": \"ConnectedValue\"}}}, {\"__index__\": 1, \"software_cond\": {\"software\": \"samtools\", \"__current_case__\": 25, \"output\": [{\"__index__\": 0, \"type\": {\"type\": \"stats\", \"__current_case__\": 0, \"input\": {\"__class__\": \"ConnectedValue\"}}}]}}, {\"__index__\": 2, \"software_cond\": {\"software\": \"picard\", \"__current_case__\": 18, \"output\": [{\"__index__\": 0, \"type\": \"markdups\", \"input\": {\"__class__\": \"ConnectedValue\"}}]}}], \"title\": \"\", \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "1.33+galaxy0",
"tool_version": "1.33+galaxy3",
"type": "tool",
"uuid": "6543abd4-0be4-4027-90e5-57644220bba3",
"when": null,
Expand Down Expand Up @@ -686,7 +685,7 @@
},
"13": {
"annotation": "Annotates detected variants",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff/5.2+galaxy1",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff/5.4+galaxy0",
"errors": null,
"id": 13,
"input_connections": {
Expand Down Expand Up @@ -738,16 +737,16 @@
"output_name": "snpeff_output"
}
},
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff/5.2+galaxy1",
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpeff/snpEff/5.4+galaxy0",
"tool_shed_repository": {
"changeset_revision": "3aae4f16ac9e",
"changeset_revision": "908f2c5fedec",
"name": "snpeff",
"owner": "iuc",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"annotations\": [\"-formatEff\", \"-classic\"], \"chr\": null, \"csvStats\": false, \"filter\": {\"specificEffects\": \"no\", \"__current_case__\": 0}, \"filterOut\": [\"-no-downstream\", \"-no-intergenic\", \"-no-intron\", \"-no-utr\", \"-no-upstream\"], \"generate_gene_stats\": false, \"generate_stats\": true, \"input\": {\"__class__\": \"ConnectedValue\"}, \"inputFormat\": \"vcf\", \"intervals\": {\"__class__\": \"RuntimeValue\"}, \"noLog\": true, \"outputFormat\": \"vcf\", \"snpDb\": {\"genomeSrc\": \"custom\", \"__current_case__\": 3, \"snpeff_db\": {\"__class__\": \"ConnectedValue\"}, \"codon_table\": \"Standard\"}, \"spliceRegion\": {\"setSpliceRegions\": \"no\", \"__current_case__\": 0}, \"spliceSiteSize\": \"2\", \"transcripts\": {\"__class__\": \"RuntimeValue\"}, \"udLength\": \"0\", \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "5.2+galaxy1",
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"type": "tool",
"uuid": "74fc9ad6-3c2c-410d-9795-540d2ae7ba6d",
"when": null,
Expand All @@ -766,7 +765,7 @@
},
"14": {
"annotation": "Extracts required fields from the VCF file",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/4.3+t.galaxy0",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/5.4.0c+galaxy0",
"errors": null,
"id": 14,
"input_connections": {
Expand All @@ -775,12 +774,7 @@
"output_name": "snpeff_output"
}
},
"inputs": [
{
"description": "runtime parameter for tool SnpSift Extract Fields",
"name": "input"
}
],
"inputs": [],
"label": "Extract using SnpSift",
"name": "SnpSift Extract Fields",
"outputs": [
Expand All @@ -794,16 +788,16 @@
"top": 644.9675784543903
},
"post_job_actions": {},
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/4.3+t.galaxy0",
"tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/snpsift/snpSift_extractFields/5.4.0c+galaxy0",
"tool_shed_repository": {
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"changeset_revision": "0abc5513f795",
"name": "snpsift",
"owner": "iuc",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"empty_text\": \".\", \"extract\": \"CHROM POS FILTER REF ALT DP AF RO AO SRP SAP EFF[*].IMPACT EFF[*].FUNCLASS EFF[*].EFFECT EFF[*].GENE EFF[*].CODON EFF[*].AA EFF[*].TRID\", \"input\": {\"__class__\": \"RuntimeValue\"}, \"one_effect_per_line\": true, \"separator\": \",\", \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_state": "{\"empty_text\": \".\", \"extract\": \"CHROM POS FILTER REF ALT DP AF RO AO SRP SAP EFF[*].IMPACT EFF[*].FUNCLASS EFF[*].EFFECT EFF[*].GENE EFF[*].CODON EFF[*].AA EFF[*].TRID\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"one_effect_per_line\": true, \"separator\": \",\", \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "4.3+t.galaxy0",
"tool_version": "5.4.0c+galaxy0",
"type": "tool",
"uuid": "af27808a-495d-4397-b248-6eb6c5954ce6",
"when": null,
Expand All @@ -820,12 +814,7 @@
"output_name": "output"
}
},
"inputs": [
{
"description": "runtime parameter for tool Collapse Collection",
"name": "input_list"
}
],
"inputs": [],
"label": "Collapse",
"name": "Collapse Collection",
"outputs": [
Expand Down Expand Up @@ -854,7 +843,7 @@
"owner": "nml",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"filename\": {\"add_name\": true, \"__current_case__\": 0, \"place_name\": \"same_multiple\"}, \"input_list\": {\"__class__\": \"RuntimeValue\"}, \"one_header\": true, \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_state": "{\"filename\": {\"add_name\": true, \"__current_case__\": 0, \"place_name\": \"same_multiple\"}, \"input_list\": {\"__class__\": \"ConnectedValue\"}, \"one_header\": true, \"__page__\": 0, \"__rerun_remap_job_id__\": null}",
"tool_uuid": null,
"tool_version": "5.1.0",
"type": "tool",
Expand All @@ -874,6 +863,7 @@
"Diploid",
"FreeBayes"
],
"uuid": "0706c30f-3bfb-42d5-bc55-a50d24824101",
"version": 1
"uuid": "dceed42d-a43a-485e-9f36-fc96628433ec",
"version": 1,
"release": "0.1.2"
}
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