diff --git a/assets/media/asaim/asaim-mt_workflow.png b/assets/media/asaim/asaim-mt_workflow.png new file mode 100644 index 000000000..59637beff Binary files /dev/null and b/assets/media/asaim/asaim-mt_workflow.png differ diff --git a/assets/media/asaim/ebi_metagenomics_workflow.png b/assets/media/asaim/ebi_metagenomics_workflow.png new file mode 100644 index 000000000..02c5c66db Binary files /dev/null and b/assets/media/asaim/ebi_metagenomics_workflow.png differ diff --git a/index-metagenomics.md b/index-metagenomics.md index 111d2be99..cd4451121 100644 --- a/index-metagenomics.md +++ b/index-metagenomics.md @@ -63,6 +63,9 @@ We then developed [several tutorials](https://galaxyproject.github.io/training-m In this tutorial the Standard Operating Procedure (SOP) for MiSeq data, developed by the creators of the Mothur software package, is perfomed within Galaxy. +- [Metatranscriptomics analysis using microbiome RNA-seq data](https://training.galaxyproject.org/training-material/topics/metagenomics/tutorials/metatranscriptomics/tutorial.html) + Metatranscriptomics analysis enables understanding of how the microbiome responds to the environment by studying the functional analysis of genes expressed by the microbiome. In this tutorial, we will analyze time-series data from a microbial community inside a bioreactor. + # Workflows To orchestrate tools and help users with their analyses, several [workflows](https://asaim.readthedocs.io/en/latest/workflows.html){:target="_blank"} are available. They formally orchestrate tools in a defined order and with defined parameters, but they are customizable (tools, order, parameters). @@ -73,7 +76,7 @@ The workflows are available in the [Shared Workflows](https://metagenomics.usega The workflow quickly produces, from raw metagenomic or metatranscriptomic shotgun data, accurate and precise taxonomic assignations, wide extended functional results and taxonomically related metabolism information -![](https://asaim.readthedocs.io/en/latest/_images/main_workflow.png) +![ASaiM main workflow](/assets/media/2018-01-17-asaim_main_workflow.png) This workflow consists of @@ -118,10 +121,24 @@ To analyze amplicon data, the **Mothur** and **QIIME** tool suites are available The tools used in the EBI Metagenomics pipeline are also available here and can be run as a [workflow](https://metagenomics.usegalaxy.eu/u/berenice/w/asaim-ebi-metagenomics-workflow-30){:target="_blank"} with the same steps as the [EBI Metagenomics pipeline (3.0)](https://www.ebi.ac.uk/metagenomics/pipelines/3.0){:target="_blank"}. -![](https://asaim.readthedocs.io/en/latest/_images/ebi_metagenomics_workflow.png) +![EBI Metagenomics Workflow](/assets/media/asaim/ebi_metagenomics_workflow.png) However, the parameters must be adjusted by the user as we could not find them in the EBI Metagenomics documentation. +## ASaiM-MT: Optimized workflow for metatranscriptomics data analysis +While the shotgun workflow is suitable for both metagenomics and metatranscriptomics datasets, we also offer an enhanced workflow aimed specifically at metatranscriptomics data. + +![ASaiM-MT workflow](/assets/media/asaim/asaim-mt_workflow.png){: width="50%"} + +The workflow is divided into 4 parts: + +1. **Preprocessing** - Process raw metatranscriptomics data to perform further analysis. +2. **Taxonomy Quantitation** - Assignment of taxonomy along with abundance values and visualization. +3. **Functional Quantitation** - metabolic assignment of identified functions and gene and pathway abundance annotation. +4. **Taxonomy-Function Quantitation** - combine taxonomy and functional quantitation values into relative abundance values at different levels such as e.g. the abundance of a pathway between phyla. + + + # References {% bibliography --cited --prefix index-metagenomics --group_by none %} diff --git a/index-microbiome.md b/index-microbiome.md new file mode 120000 index 000000000..295291ba5 --- /dev/null +++ b/index-microbiome.md @@ -0,0 +1 @@ +index-metagenomics.md \ No newline at end of file