diff --git a/mite_data/data/ac03b692-a6a9-11f1-b4b1-cf84655b59e7.json b/mite_data/data/ac03b692-a6a9-11f1-b4b1-cf84655b59e7.json
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@@ -0,0 +1,62 @@
+{
+    "accession": "MITE9999999",
+    "status": "pending",
+    "comment": "NgvF is a homologue of AoiQ-Methyltransferase domain and can perform methylation to produce dichlorodiaporthin",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2026-09-02",
+            "contributors": [
+                "0009-0008-8086-5325"
+            ],
+            "reviewers": [
+                "BBBBBBBBBBBBBBBBBBBBBBBB"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "NgvF",
+        "description": "O-methyltransferase",
+        "references": [
+            "doi:10.1021/jacs.1c02868"
+        ],
+        "databaseIds": {
+            "uniprot": "UPI0009D33686",
+            "genpept": "OQE91620.1"
+        },
+        "cofactors": {
+            "organic": [
+                "SAM"
+            ]
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Methylation"
+            ],
+            "description": "NgvF, a homologue of AoiQ-MT domain catalyzes methylation to form a O-6-methylated structure, dichlorodiaporthin",
+            "reactionSMARTS": "[#8:1]-[#6:2]1:[#6:19]:[#6:17](-[#8:18]):[#6:16]2:[#6:4](:[#6:5]:[#6:6](:[#8:13]:[#6:14]:2=[#8:15])-[#6:7]-[#6@@:8](-[#6:10](-[Cl:12])-[Cl:11])/[#8:9]):[#6:3]:1>>[#8:1](-[#6])-[#6:2]1:[#6:19]:[#6:17](-[#8:18]):[#6:16]2:[#6:4](:[#6:5]:[#6:6](:[#8:13]:[#6:14]:2=[#8:15])-[#6:7]-[#6@@:8](-[#6:10](-[Cl:12])-[Cl:11])/[#8:9]):[#6:3]:1",
+            "reactions": [
+                {
+                    "substrate": "O=c1oc(C[C@H](O)C(Cl)Cl)cc2cc(O)cc(O)c12",
+                    "products": [
+                        "COc1cc(O)c2c(=O)oc(C[C@H](O)C(Cl)Cl)cc2c1"
+                    ],
+                    "isIntermediate": false,
+                    "description": "AoiQ-MT domain can catalyze both O-6 and O-8 methylations to produce 8-methyldichlorodiaporthin, whereas NgvF can catalyze only O-6 methylation to produce dichlorodiaporthin"
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "In vitro assay"
+                ],
+                "references": [
+                    "doi:10.1021/jacs.1c02868"
+                ]
+            }
+        }
+    ]
+}
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