diff --git a/s3transfer/crt.py b/s3transfer/crt.py
index b11c3682..f2897696 100644
--- a/s3transfer/crt.py
+++ b/s3transfer/crt.py
@@ -11,6 +11,7 @@
 # ANY KIND, either express or implied. See the License for the specific
 # language governing permissions and limitations under the License.
 import logging
+import os
 import re
 import threading
 from io import BytesIO
@@ -67,6 +68,22 @@ def acquire_crt_s3_process_lock(name):
     # lock is set as a global so that it is not unintentionally garbage
     # collected/released if reference of the lock is lost.
     global CRT_S3_PROCESS_LOCK
+
+    def after_in_child():
+        global CRT_S3_PROCESS_LOCK
+        if CRT_S3_PROCESS_LOCK is not None:
+            # the lock is not belong to the forked child process.
+            # Release the lock after fork in child process.
+            CRT_S3_PROCESS_LOCK.release()
+            CRT_S3_PROCESS_LOCK = None
+
+    # Check if we've already registered using a function attribute
+    if not getattr(
+        acquire_crt_s3_process_lock, '_fork_handler_registered', False
+    ):
+        os.register_at_fork(after_in_child=after_in_child)
+        acquire_crt_s3_process_lock._fork_handler_registered = True
+
     if CRT_S3_PROCESS_LOCK is None:
         crt_lock = awscrt.s3.CrossProcessLock(name)
         try: