diff --git a/Formula/r/ruff.rb b/Formula/r/ruff.rb
index 7b818337c980..85381e479444 100644
--- a/Formula/r/ruff.rb
+++ b/Formula/r/ruff.rb
@@ -1,18 +1,17 @@
 class Ruff < Formula
   desc "Extremely fast Python linter, written in Rust"
   homepage "https://docs.astral.sh/ruff/"
-  url "https://github.com/astral-sh/ruff/archive/refs/tags/0.16.4.tar.gz"
-  sha256 "8e26878a97a0c7f2b364bac11b585b793b8913b14b850c0922d27d4d06de5173"
+  url "https://github.com/astral-sh/ruff/archive/refs/tags/0.16.5.tar.gz"
+  sha256 "c447968b1e608450c973d441fa87f949f676064aec2db458b557e8222a4eb252"
   license "MIT"
   head "https://github.com/astral-sh/ruff.git", branch: "main"
 
   bottle do
-    sha256 cellar: :any_skip_relocation, arm64_tahoe:   "cff7690a268916bbe459ef05c7d4f776c0608694d226d181e086e9ea7ff30d77"
-    sha256 cellar: :any_skip_relocation, arm64_sequoia: "6477c045d41abe1ba33b3d3b0ff08e6dac0088d987286ec2e98523044c034bfc"
-    sha256 cellar: :any_skip_relocation, arm64_sonoma:  "97d26d7793ca54c41c726f93d3b2fa8480c1232449af0198ec3b4e1fd63c42bb"
-    sha256 cellar: :any_skip_relocation, sonoma:        "40ee8371f595615a1ad33b22e98569bcdcdf47d027691d34c414cf17aada6e53"
-    sha256 cellar: :any,                 arm64_linux:   "d12117d11445def19ce175a26f7dfc91b2b61ea7c3867dd34cb8f502a0d8b717"
-    sha256 cellar: :any,                 x86_64_linux:  "d46ec6418f6cdce18fb478a16233c0fb012b7d3eb1ceb291284d00f28e9e6f36"
+    sha256 cellar: :any_skip_relocation, arm64_tahoe:   "9347fbb908789c5f2e4a8390989660a1b54ad9e22acdadfda642507f28b6eae1"
+    sha256 cellar: :any_skip_relocation, arm64_sequoia: "54f751a405163096521c58dba1937a4e8ff32e0971c28d56450481d5c125f78a"
+    sha256 cellar: :any_skip_relocation, arm64_sonoma:  "49488c0be3ff035e6918c9fe2ec1cc7a1e8ce5aebf5caeb9bfcdd1431f84be3a"
+    sha256 cellar: :any,                 arm64_linux:   "7d207e6d081aae5eef876376a69ac4c3ee52a185b9d6e387874fba60ea1e2704"
+    sha256 cellar: :any,                 x86_64_linux:  "fcd2b4565b7f9b2fd31796d8d004a01c440cc860a52e6e0d7f31835ad9915d3f"
   end
 
   depends_on "rust" => :build